IDENTIFIKASI PROFIL BAKTERI DAN TINGKAT KERENTANAN VARIETAS RUMPUT LAUT (KAPPAPHYCUS SPP. DAN SARGASSUM SP.) TERHADAP PENYAKIT ICE-ICE DI PERAIRAN KABUPATEN ALOR

Donny Mercys Bessie; Alfred G. O. Kase; Wilson L. Tisera; Jusuf Aboladaka; Nina Jeny Lapinangga

doi:10.63754/jcn.v3i4.185

Authors

Donny Mercys Bessie Universitas Kristen Artha Wacana
Alfred G. O. Kase Universitas Kristen Artha Wacana Kupang, Indonesia
Wilson L. Tisera Universitas Kristen Artha Wacana Kupang, Indonesia
Jusuf Aboladaka Universitas Kristen Artha Wacana Kupang, Indonesia
Nina Jeny Lapinangga Politeknik Pertanian Negeri Kupang, Indonesia

DOI:

https://doi.org/10.63754/jcn.v3i4.185

Keywords:

Ice-ice, Vibrio alginolyticus, Kappaphycus alvarezii, Alor Regency, Pathogenicity

Abstract

Ice-ice disease is a major threat to seaweed cultivation productivity in Alor Regency. This study aims to identify the causative agent of Ice-ice disease through microbiological analysis (presumptive and abundance tests) and evaluate the susceptibility levels of various seaweed varieties. Samples tested included Kappaphycus alvarezii (green and brown strains), Kappaphycus striatum, and Sargassum crassifolium. Presumptive test results showed that all isolates were Gram-negative, catalase-positive, and oxidase-positive. Species identification showed the dominance of Vibrio alginolyticus as the main pathogen found in almost all samples. K. alvarezii Green Strain was identified as the most susceptible variety with the highest infection rate (total estimated ~1.8 colonies/mL) due to coinfection with Vibrio alginolyticus, Vibrio sp., and Aeromonas faecalis. In contrast, K. striatum showed the best resistance with the lowest bacterial abundance (0.3 colonies/mL). The presence of V. alginolyticus in Sargassum crassifolium indicates its role as a natural reservoir. These findings emphasize the need for variety-based and biosecurity-based cultivation management strategies in Alor.

References

Bessie, D. M., Lapinangga, N. J., Dawa, U. P. L., Tisera, W. L., Emola, I. J., Kase, A. G. O., Ndolu, H., Aboladaka, J., & Ena, Z. (2022). Sequence Analysis of Seaweed Farming in Hundihuk Village, Rote-Ndao District. Ecology, Environment and Conservation Paper, 28(2), S104–S109. https://doi.org/10.53550/EEC.2022.v28i05s.019

Bessie, D. M., Mushab, Adri, T., & Marlita. (2025). Kajian Pembaharuan Pengelolaan Perikanan dengan Pendekatan Ekosistem di Kabupaten Alor Provinsi Nusa Tenggara Timur. Yayasan WWF Indonesia.

Bessie, D. M., Tisera, W. L., Dawa, U. P. L., Kase, A. G. O., Tisera, V. R. T., Lapinangga, N. J., & Adisasmito, S. (2024). Analysis of bacteria caused ice-ice disease in seaweed through polyculture in Dengka Island, Rote Ndao Regency. AIP Conference Proceedings, 3073(1), 030013. https://doi.org/10.1063/5.0193767

Bessie, D. M., Wilson, L. T., Dawa, U. P. L., Kase, A. G. O., Herawaty, S., & Lapinangga, N. J. (2025). Analysis of suitability area and carrying capacity of seaweeds farming in Holulai Village, Rote Ndao Regency. IOP Conference Series: Earth and Environmental Science, 1496, 012031. https://doi.org/10.1088/1755-1315/1496/1/012031

Boro, A., Anggawarmasse, I., Tanadoy, A., Bessie, D. M., Rusydi, Ninef, J., Tisera, W. L., & Toruan, L. (2022). Grand Design Kelautan dan Perikanan Provinsi Nusa Tenggara Timur. Dinas Kelautan dan Perikanan Provinsi NTT.

Prasetyo, E. N., Permanasari, Y., Basyirah, A., Fitriya, S. A., Jadid, N., Zullaikah, S., Ekawati, I., Isdiantoni, & Koentjoro, M. P. (2024). Aquaculture infection of Kappaphycus alvarezii by Vibrio alginolyticus causing ice-ice disease. Biodiversitas Journal of Biological Diversity, 25(11). 4086–4094. https://doi.org/10.13057/biodiv/d251108

Tahiluddin, A., & Terzi, E. (2021). Ice-Ice Disease in Commercially Cultivated Seaweeds Kappaphycus spp. and Eucheuma spp.: A Review on the Causes, Occurrence, and Control Measures. Marine Science and Technology Bulletin, 10(3), 234–243. https://doi.org/10.33714/masteb.917788

Tahiluddin, B. A., & Damsik, S. U. (2023). Prevalence of ice-ice disease in Kappaphycus spp. and Eucheuma denticulatum farms in Sibutu, Tawi-Tawi, Philippines. Aquaculture Studies, 23(5), 1–7. https://doi.org/10.4194/AQUAST1137

Tuhumury, N. C., Sahetapy, J. M. F., & Matakupan, J. (2024). Isolation and identification of bacterial pathogens causing ice-ice disease in Eucheuma cottonii seaweed at Seira Island Waters, Tanimbar Islands District, Maluku, Indonesia. Biodiversitas Journal of Biological Diversity, 25(3), 964–970. https://doi.org/10.13057/biodiv/d250308

Ward, G. M., Kambey, C. S. B., & Faisan, J. P. (2022). Ice-Ice disease: An environmentally and microbiologically driven syndrome in tropical seaweed aquaculture. Reviews in Aquaculture, 14(1), 414–439. https://doi.org/10.1111/raq.12606